Combining lipoic acid to methylene blue reduces the Warburg effect in CHO cells: From TCA cycle activation to enhancing monoclonal antibody production

Ce travail (Montégut et al. 2020), fait en collaboration avec le Dr. Laurent Schwartz M.D. et le Dr. Jorgelindo Da Veiga Moreira à Polytechnique Montréal dans le groupe du Professeur Mario Jolicoeur, montre qu’une vieille molécule comme le Bleu de Méthylène lève l’effet Warburg. Ce traitement s’accompagne d’un ralentissement de la croissance cellulaire. L’effet inhibiteur de croissance est d’autant plus puissant que le Bleu de Méthylène est administré avec de l’acide lipoïque. Ceci conforte l’addition du Bleu de Méthylène au traitement métabolique. Ce travail a été publié dans une revue internationale à comité de lecture.

Léa Montégut, Pablo César Martínez-Basilio, Jorgelindo da Veiga Moreira, Laurent Schwartz, Mario Jolicoeur
Published: April 16, 2020 https://doi.org/10.1371/journal.pone.0231770

Abstract

The Warburg effect, a hallmark of cancer, has recently been identified as a metabolic limitation of Chinese Hamster Ovary (CHO) cells, the primary platform for the production of monoclonal antibodies (mAb). Metabolic engineering approaches, including genetic modifications and feeding strategies, have been attempted to impose the metabolic prevalence of respiration over aerobic glycolysis. Their main objective lies in decreasing lactate production while improving energy efficiency. Although yielding promising increases in productivity, such strategies require long development phases and alter entangled metabolic pathways which singular roles remain unclear. We propose to apply drugs used for the metabolic therapy of cancer to target the Warburg effect at different levels, on CHO cells. The use of α-lipoic acid, a pyruvate dehydrogenase activator, replenished the Krebs cycle through increased anaplerosis but resulted in mitochondrial saturation. The electron shuttle function of a second drug, methylene blue, enhanced the mitochondrial capacity. It pulled on anaplerotic pathways while reducing stress signals and resulted in a 24% increase of the maximum mAb production. Finally, the combination of both drugs proved to be promising for stimulating Krebs cycle activity and mitochondrial respiration. Therefore, drugs used in metabolic therapy are valuable candidates to understand and improve the metabolic limitations of CHO-based bioproduction.

 

 

Fig 1. Growth and viability responses of CHO cells to various doses of α-lipoic acid (Α-LA) and methylene blue (MB).
α-LA was tested at 10 μM, 20 μM, 50 μM, 100 μm, 200 μM and 500 μM (A) and MB was tested at 10 nM, 100 nM, 500 nM, 1 μM and 10 μM. Growth and viability curves are presented as means ± SEM (n = 3). Specific growth rates were calculated by linear regression during the exponential growth phase, from 0 to 72 h. Statistical significance was determined by one-way ANOVA versus the control culture.
https://doi.org/10.1371/journal.pone.0231770.g001

The addition of MB showed no growth inhibition until 500 nM, with cultures at 10 nM, 100 nM and 500 nM behaving similarly to the control (average μ = 0.043 ± 0.002 h-1, Fig 1B). At 1 μM, a minor decrease of the cells specific growth rate was observed (μ = 0.041 ± 0.003 h-1, p = 0.56). Of interest, the viability was maintained at the end of the culture, with 92 ± 1% at 120 h when treated with 1 μM MB compared to 77 ± 3% for the control culture. However, the cells growth rate was significantly reduced at 10 μM (μ = 0.022 ± 0.003 h-1, p < 0.001). Therefore, and following the same criterion as for α-LA, a MB concentration of 1 μM was used in the remainder of the study.

α-LA and MB have distinct significant metabolic effects


The effect of the drugs on cell metabolism was then characterized in shake flask cultures. Drugs were assayed alone as well as combined, and compared to three controls: non-treated (control), treated only with the vehicle used for α-LA administration (0.1% ethanol, control + vehicle) and treated with a known PDH activator (DCA 5 mM). As inferred by our previous observations, similar cell growth and viability behaviors were observed in all cultures (Fig 2A), with a specific growth rate of μ = 0.040 ± 0.002 h-1 and viability higher than 95% until 96 h, except for 100 μM α-LA where cell growth was affected (μ = 0.033 ± 0.002 h-1, p = 0.01, Fig 2A–3). The positive impact of MB on viability was confirmed, with levels of 84 ± 1% for 1 μM MB and 82 ± 3% when combined with 20 μM Α-LA at 120 h, compared to 73 ± 4% for the control (Fig 2A–2).

Fig 2. Metabolic responses after drug administration.
https://doi.org/10.1371/journal.pone.0231770.g002
All drugs were added to the culture medium prior to inoculation, with the following conditions: control, 0.1% ethanol (control + vehicle), 5 mM DCA, 20 μM α-LA, 100 μM α-LA, 1 μM MB and 20 μM α-LA combined with 1 μM MB. (A) Cellular growth, viability and specific growth rates were compared to the control. (B) The glucose (GLC) consumption and lactate (LAC) production rates were compared by calculating their ratio (YLAC/GLC). This yield was taken from 0 to 48 h (exponential growth phase) and from 48 to 120 h (late phase), then used to quantify the glycolytic fluxes. (C) Glutamine (GLN) consumption rates were compared to glutamate (GLU) production rates before glutamine depletion (0–72 h), the resulting yield (YGLU/GLN) quantifies the share of glutamine directed to anaplerosis.

Aerobic glycolysis.


Cells glycolytic metabolism was analyzed by comparing glucose consumption to lactate production (Fig 2B). Glucose specific uptake rate (qGLC) and lactate specific production rate (qLAC) were determined in two distinct metabolic phases, taking into account a metabolic shift observed at 48 h. The first phase was calculated from 0–48 h during the exponential growth phase, where both glucose consumption and lactate production fluxes stayed at high levels, with qGLC = 0.22 ± 0.01 μmol/106cells/h and qLAC = 0.36 ± 0.02 μmol/106cells/h for the control group (S1 Fig). The second phase, i.e. late growth phase (48–120 h), was characterized by lower fluxes, with a decrease of 79% for qGLC and 90% for qLAC in the control culture. Similar trends were observed in all conditions (S1 Fig). The YLAC/GLC yield (- qLAC/qGLC) shows that in all conditions most of the uptake glucose undergoes aerobic glycolysis during exponential growth, while this phenomenon is reduced by half during the late growth phase (Fig 2B–3). No significant differences were found when cells were treated with drug vehicle (0.1% ethanol) alone, 20 μM α-LA or its positive control 5 mM DCA. However, 100 μM α-LA resulted in a reduced contribution of aerobic glycolysis, especially during the late growth phase (YLAC/GLC = 0.51 ± 0.01 mol/mol vs. 0.84 ± 0.05 mol/mol for the control). At 1 μM, MB showed to decrease YLAC/GLC both alone and in combination with 20 μM α-LA for the first 48 h, with -19% and -23% versus the control, respectively, and to be similar to the control thereafter.

Glutaminolysis.


Before glutamine depletion, observed at ~72 h in all conditions, all treatments showed strong effects on the glutaminolysis pathway, evaluated from the YGLU/GLN yield (- qGLU/qGLN) at 0-72 h (Fig 2C). Glutaminolysis refers to the efficient use of glutamine, second carbon and nitrogen source, incorporated in the TCA cycle. Cells treated with 5 mM DCA showed a 24% increase of YGLU/GLN, and thus a decreased glutaminolysis phenomenon. However, significant YGLU/GLN increases were observed at 20 μM and 100 μM α-LA, with + 43% and + 48% respectively (Fig 2C–3). It was also observed that supplementing the culture with the drug vehicle (0.1% ethanol) alone caused a slight increase of + 21% in YGLU/GLN, compared to control. Interestingly, the addition of MB at 1 μM showed to favor glutaminolysis with – 24% measured for YGLU/GLN. Finally, when used in combination with MB, the effect of α-LA was predominant with a + 38% increase in YGLU/GLN (Fig 2C–3). Therefore, α-LA and DCA, both drugs known to activate the pyruvate dehydrogenase (PDH) and thus stimulate pyruvate entry into mitochondria, decreased the entry of glutamine in the TCA cycle, while MB increased glutaminolytic anaplerosis.

Drug combination promotes cells OxPhos


The cell specific oxygen consumption rate (qO2) observed for the control culture at 24 h, with qO2 = 0.22 ± 0.02 μmolO2/106cells/h, was similar to previous data obtained with the same cell line [53]. While being maintained during exponential growth phase, qO2 then constantly and strongly decreased (Fig 3A). Such trend was observed in both respiration and leak components of the global qO2 (Fig 3B and 3C). The use of 5 mM DCA increased qO2 and qO2,resp by up to 27% and 38% at 24 h, respectively, compared to control. However, this effect was only maintained for the growth phase, then qO2 values decreased to control level. A concentration of 100 μM α-LA did not initially increase qO2 but, unlike DCA, it kept the respiration level constant until 120 h (Fig 3A), with an approximate 1:1 ratio between respiration and leak (Fig 3B and 3C). No such effect was observed with α-LA at 20 μM or MB at 1 μM, although their combination allowed to partially maintain cell respiration to the end of the culture. At 120 h, combined α-LA and MB led to a qO2,resp value 5.6 times higher than the control (Fig 3B), with a qO2,leak equal to that of control (Fig 3C). Of interest, the combination of the two drugs also perturbed the distribution between leak and respiration at 24 h since, although total qO2 remained unchanged, the leak accounted for 70% of global qO2 instead of 50% for the control (Fig 3C).

Fig 3. Impact of the various treatments on oxygen consumption.
https://doi.org/10.1371/journal.pone.0231770.g003
Specific oxygen consumption rates (qO2) were measured for the different treatments with and without the ATP-synthase inhibitor oligomycin A (1 μM) in order to determine the total qO2 (A), its share due to leak qO2,leak (C) and the remaining share due to mitochondrial respiration qO2,resp (B). All values were normalized to the qO2 of their control at 24 h to allow for comparison.

Drugs affect mitochondrial membrane potential and oxidative stress level


The mitochondrial activity was assessed by FACS following two different markers: the mitochondrial membrane potential (MMP), stained by Rhodamine123, and the reactive oxygen species (ROS) generation at the membrane, stained by MitoSOX. We chose the MMP and ROS values of control at 24 h as references for all conditions and compared their evolution to these designated references. The MMP of the control increased with time up to 3-fold after the exponential growth phase (Fig 4A), a trend opposite to that of cell respiration. The addition of 0.1% ethanol (control + vehicle) resulted in a greater but maintained MMP at the end of the culture. Pronounced increases of MMP were observed in both cultures treated with DCA at 5 mM and α-LA at 100 μM, with respective increases of 9.8 and 9.1 times the reference, measured at 120 h. In contrast, 20 μM α-LA culture maintained a low MMP, under 80% of that of the reference. Finally, the addition of MB did not affect MMP, except when combined to 20 μM α-LA where an initial burst was observed at 3.2 times the reference level, while remaining at control level until the end of the culture.

Fig 4. Mitochondrial membrane potential and reactive oxygen species (ROS) levels induced by the drugs.
Mean fluorescence intensity (MFI) was measured by FACS after staining with Rhodamine123 (A) for the mitochondrial membrane potential, and with MitoSOX (B) for the levels of superoxide ions located at the mitochondria. All values are presented as means ± SEM with arbitrary units (normalized versus the MFI of the control at 24 h).
https://doi.org/10.1371/journal.pone.0231770.g004
When functioning normally, the electron transport chain (ETC) generates ROS, among which superoxide ions can be stained by the MitoSOX fluorescent dye. The control, drug vehicle and 5 mM DCA (to a lesser extent) conditions showed similar trends, with stable levels at 24 and 72 h, and 1.5 to 2-fold increase at 120 h (Fig 4B). In agreement with their high mortality levels, cells treated with 100 μM of α-LA excessively generated mitochondrial ROS. Finally, at 120 h, instead of the doubling observed for the control, 20 μM α-LA, 1 μM MB and their combination showed decreasing ROS levels with respectively 0.9, 0.6 and 0.5 times the reference value (Fig 4B).
 

MB significantly increases the final monoclonal antibody titer


Maximum mAb titers were reached at 96 h and decreased afterwards (Fig 5A), although not exactly following viability trends (Fig 1). A similar maximum value of 49 ± 3 mg/L was measured for the control, the 20 μM α-LA and 5 mM DCA conditions. The addition of 0.1% ethanol (control + vehicle) resulted in a final production reduction of 20% (Fig 5B). The use of 100 μM α-LA decreased the maximal titer by 67%, and it was not the result of the presence of ethanol alone (p < 0.001, one-way ANOVA vs. control + vehicle). Notably, the addition of MB at 1 μM stimulated the mAb production (+24 ± 5%, p = 0.0013). A positive but non-significant increase (+7 ± 3%, p = 0.25) was also detected when MB was combined to 20 μM α-LA.

Fig 5. Monoclonal antibody (mAb) production and variation of the maximum mAb titer in the extracellular medium for the various drug treatments versus control.
(A) Product titer was determined by ELISA and (B) its effect on mAb production is presented as the percentage of variation versus control.
https://doi.org/10.1371/journal.pone.0231770.g005

 

Discussion

Up-regulation of pyruvate dehydrogenase can lead to OxPhos saturation


Two of the three drugs tested (i.e. α-LA and DCA) target the PDH enzyme, which converts pyruvate to mitochondrial acetyl co-enzyme A (AcCoA) rather than to extracellular lactate. However, the expected decrease of global lactate production and of YLAC/GLC has only been observed at 100 μM α-LA,while it was not significant at 20 μM α-LA nor 5 mM DCA (Fig 2B). At 20 μM α-LA, the increase of qLAC was counterbalanced by a slight (but not significant) increase of qGLC (S1 Fig). Although both specific rates increased at 100 μM α-LA, the increase was higher for qGLC than for qLAC, which explains a lower YLAC/GLC. With no significant effect on qLAC or qGLC, our results with DCA differ from Buchsteiner et al. (2018), which may underly some cell line differences. However, both drugs (DCA and α-LA alone or in combination with MB) reduced glutaminolysis (Fig 2C), the main anaplerotic pathway in CHO cells [54, 55], a result mostly due to an increased qGLU (S1 Fig). Martínez et al. (2013) report that CHO cells maintain constant TCA fluxes by reducing glutaminolysis when other anaplerotic fluxes are activated during the glycolysis/OxPhos switch. These results suggest that α-LA and DCA-treated cells may increase their YGLU/GLN ratio in order to compensate for an increased anaplerosis. Indeed, α-LA is known to activate multiple entry-point enzymes to the TCA cycle [56]. A similar conclusion was drawn by Zagari et al. (2013) who used a model of restricted mitochondrial oxidative capacity to explain the codependency of glutamine and lactate metabolisms.

Evaluating the drugs effect on mitochondrial activity homeostasis requires looking at respiratory data. In our work, the enhanced TCA activity from 5 mM DCA was confirmed by an increased total qO2 during exponential growth (0–72 h, Fig 3). However, these increased TCA fluxes resulted, at 120 h, in a mitochondrial imbalance with proton accumulation at the membrane (Rho123, Fig 4A) and a reduction of cellular respiration (Fig 3B). These results agree with the lower ATP concentrations at 5 mM DCA which were previously reported by Buchsteiner et al. (2018). At 100 μM α-LA, the stimulation of TCA cycle activity resulted in a maintained oxygen consumption rate from 0 to 120 h. However, as for our positive DCA control, a significant proton accumulation was observed at the mitochondrial membrane. This mitochondrial saturation at 100 μM α-LA coincided with increased levels of mitochondrial ROS (Fig 4), and proton leak flux (Fig 3C), indicating extreme levels of stress coherent with the observed decrease in cell viability. Also from using Rhodamine123 staining, Hinterkörner et al. (2007) proposed aerobic glycolysis as a mitochondrial pressure relief mechanism, which can be triggered from a high mitochondrial membrane potential. Interestingly, the addition of 20 μM α-LA did not alter the respiration and proton leak rate profiles, while maintaining low mitochondrial membrane potential and ROS levels. The mitochondrial activity and redox balance are strongly dependent on α-LA. It does not only have antioxidant properties but it also acts as cofactor of many mitochondrial enzymes in addition to its action on PDH [57]. For instance, the regulation of complex I production of superoxide anion through its interaction with 2-oxoglutarate dehydrogenase [56] can account in part for the restriction in ROS production (Fig 4). To sum up, α-LA is efficient to manage TCA replenishment and positively regulate the mitochondrial function, but at high concentration such as 100 μM and above, significant changes in mitochondrial metabolism induce damageable stress levels.

Methylene blue enhances the mitochondrial capacity


MB at 1 μM clearly enhanced mitochondrial capacity, a conclusion supported by a coherent set of coordinated effects including lower lactate yield (i.e. more glycolytic flux to TCA cycle), higher glutaminolysis (i.e. more glutamate flux to TCA cycle), control level qO2 and mitochondrial membrane potential, and lower ROS level. MB is a potent redox exchanger acting as an electron shuttle in the mitochondria, bypassing complexes I to III of the ETC and resulting in decreased ROS production [46, 58]. High levels of mitochondrial ROS are associated to high proton leak rates in order to dampen ROS production, thus decreasing ATP synthesis [59]. From these results, we hypothesize that 1 μM MB induces an oxidoreductive “sink” at ETC that pulls on the various anaplerotic pathways to feed the TCA cycle, explaining decreased lactate and glutamate secretion rates (S1 Fig). Such enhanced mitochondrial activity can account for the observed increase in mAb production (Fig 5). Interestingly, coupling α-LA to MB combines the effects of each drug, with a reduced aerobic glycolysis and low ROS levels. The signs of healthy mitochondria are confirmed by the significantly higher qO2 at the end of the culture (Fig 3), although it only translated into a 7 ± 3% increase in mAb production.
 

Conclusion


Our results provide further evidence on the use of metabolic approaches to understand and overcome Warburg effect-related limitations on mAb production by CHO cells.By up-regulating PDH, the α-lipoic acid (α-LA) drug proved efficient at redirecting anaplerotic fluxes towards mitochondria thus increasing TCA activity. However, α-LA above 100 μM disturbs the tightly regulated redox status at the ETC, inducing important stress signals, while 20 μM maintains a minimal stress level. Of interest, the use of methylene blue (MB) at 1 μM showed promising results with increased mitochondrial activity under minimal stress level, and increased mAb production. Although the combination of MB and α-LA led to a less pronounced increase of mAb production than using MB only, it improved cellular respiration. The coordinated actions of pushing on pyruvate entry into mitochondria (α-LA) and pulling on anaplerotic pathways feeding the TCA cycle, while maintaining low ROS level (MB), revealed regulations that confirm the metabolic similarities between CHO and cancer cells.

At the molecular level, metabolic changes can impact mAb quality, i.e. the glycosylation profile and biological activity. Further dedicated studies would be required to identify optimal lipoic acid and methylene blue concentrations and ratios to preserve the mAb molecular properties. We chose to focus on the net production of antibody as it reflects the general metabolic state of the cell. Using this criterium, we showed that, even more than the imbalance between glycolysis and respiration, the mitochondrial capacity was critical for productivity in this CHO cell line. Altogether, the metabolic drugs originating from human therapy proved to be a convenient and efficient tool to study and direct the metabolic regulations of CHO-based bioprocesses.
 

Supporting information

Combining lipoic acid to methylene blue reduces the Warburg effect in CHO cells: From TCA cycle activation to enhancing monoclonal antibody production
Showing 1/2: pone.0231770.s001.docx

 

Fig S1. Specific consumption and production rates
Specific consumption and production rates of glucose (A), lactate (B), glutamine (C) and glutamate (D) were measured in the extracellular medium for the various drug treatments. Glycolytic specific rates qGLC and qLAC were calculated on 0-48 h and 48-120 h based on the metabolic shift observed at 48 h. Glutaminolytic rates qGLN and qGLU were calculated before (0-72 h) and after (72-120 h) glutamine depletion. All conditions were statistically compared to the control by one-way ANOVA.

 

Acknowledgments


Authors wish to thank Frédéric Bouillaud for helpful discussions.

 

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  • 1 856 547 visites

Léa Montégut

Roles Conceptualization, Data curation, Investigation, Methodology,
Writing – original draft, Writing – review & editing



Affiliation
Department of Chemical Engineering, Research Laboratory

in Applied Metabolic Engineering, École Polytechnique de Montréal,
Montréal, Québec, Canada

Pablo César Martínez-Basilio

Roles Conceptualization, Methodology



Affiliation
Department of Chemical Engineering, Research Laboratory

in Applied Metabolic Engineering, École Polytechnique de Montréal,
Montréal, Québec, Canada

Jorgelindo da Veiga Moreira

Roles Conceptualization



Affiliation
Department of Chemical Engineering, Research Laboratory
in Applied Metabolic Engineering, École Polytechnique de Montréal,
Montréal, Québec, Canada

Laurent Schwartz

Roles Conceptualization


Affiliation Assistance Publique des Hôpitaux de Paris, Paris, France

Mario Jolicoeur

Roles Conceptualization, Formal analysis, Funding acquisition,
Investigation, Methodology, Project administration, Supervision, Writing – original draft, Writing –
review & editing


mario.jolicoeur@polymtl.ca



Affiliation
Department of Chemical Engineering, Research Laboratory
in Applied Metabolic Engineering, École Polytechnique de Montréal,
Montréal, Québec, Canada

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